Five days prior to microwave exposure, baseline data on daily flight activity, as well as temperature within the colonies and anechoic chambers, were taken to verify that both colonies and chambers were equivalent prior to the onset of exposure. Just prior to initiation of the exposure, [1] the comb of honey that had been placed in each hive was replaced with a full comb of honey to insure adequate food reserves for the duration of exposure, [2] one empty comb was added to each hive to stimulate egg laying, [3J both hives were weighed, [4] the number and distribution of food and brood cells in all combs were recorded. Then the four frames of comb in each hive were arranged from front (entrance end) to back as follows: [1] comb of brood from eggs laid 20 days prior to start of exposure, [2] empty comb, [3] comb of brood from eggs laid 9 days prior to start of exposure, and [4] comb of honey.
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